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nonlinear regression curve fitting to a four-parameter logistic equation with graphpad prism 9 software  (GraphPad Software Inc)

 
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    GraphPad Software Inc nonlinear regression curve fitting to a four-parameter logistic equation with graphpad prism 9 software
    Nonlinear Regression Curve Fitting To A Four Parameter Logistic Equation With Graphpad Prism 9 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nonlinear regression curve fitting to a four-parameter logistic equation with graphpad prism 9 software/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    nonlinear regression curve fitting to a four-parameter logistic equation with graphpad prism 9 software - by Bioz Stars, 2026-03
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    Identification of peptides containing a potential AIM sequence in TgAtg3 by AlphaScreen competition assay. A, schematic of the AlphaScreen assay. Biotinylated TgAtg8 and His-tag TgAtg3 are immobilized on streptavidin-coated donor beads and nickel-chelated acceptor beads, respectively. Excitation of donor beads produces singlet oxygen. Interaction between TgAtg8 and TgAtg3 brings donor and acceptor beads into close proximity, allowing energy from singlet oxygen to be transferred to the acceptor beads and produce a light signal. Addition of untagged competitor peptides will reduce the signal intensity. B, competition for binding to biotinylated TgAtg8 domain by untagged peptides of TgAtg3. Each competitor was tested in triplicate, with a representative data set shown. IC50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software. Data were obtained from one of four independent experiments.
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    Identification of peptides containing a potential AIM sequence in TgAtg3 by AlphaScreen competition assay. A, schematic of the AlphaScreen assay. Biotinylated TgAtg8 and His-tag TgAtg3 are immobilized on streptavidin-coated donor beads and nickel-chelated acceptor beads, respectively. Excitation of donor beads produces singlet oxygen. Interaction between TgAtg8 and TgAtg3 brings donor and acceptor beads into close proximity, allowing energy from singlet oxygen to be transferred to the acceptor beads and produce a light signal. Addition of untagged competitor peptides will reduce the signal intensity. B, competition for binding to biotinylated TgAtg8 domain by untagged peptides of TgAtg3. Each competitor was tested in triplicate, with a representative data set shown. IC50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software. Data were obtained from one of four independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of the molecular mechanism of the autophagy-related Atg8–Atg3 protein interaction in Toxoplasma gondii

    doi: 10.1074/jbc.RA118.002614

    Figure Lengend Snippet: Identification of peptides containing a potential AIM sequence in TgAtg3 by AlphaScreen competition assay. A, schematic of the AlphaScreen assay. Biotinylated TgAtg8 and His-tag TgAtg3 are immobilized on streptavidin-coated donor beads and nickel-chelated acceptor beads, respectively. Excitation of donor beads produces singlet oxygen. Interaction between TgAtg8 and TgAtg3 brings donor and acceptor beads into close proximity, allowing energy from singlet oxygen to be transferred to the acceptor beads and produce a light signal. Addition of untagged competitor peptides will reduce the signal intensity. B, competition for binding to biotinylated TgAtg8 domain by untagged peptides of TgAtg3. Each competitor was tested in triplicate, with a representative data set shown. IC50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software. Data were obtained from one of four independent experiments.

    Article Snippet: IC 50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software.

    Techniques: Sequencing, Amplified Luminescent Proximity Homogenous Assay, Competitive Binding Assay, Binding Assay, Software